Wound Healing Activity of Extract of Medicinal Plant Pistacia Integrrima

Abstract

The provided text discusses the wound healing potential of Pistacia integerrima plant extract in the context of addressing chronic wounds. The study focuses on evaluating the wound healing activity of the plant material through in vitro and in vivo analyses. Key findings include the presence of various phytochemicals in the plant, such as carbohydrates, proteins, saponins, flavonoids, tannins, and phenols.

The total phenol and flavonoid content were quantified as 0.316 and 0.244, respectively. In the in vivo setting, the hydroalcoholic extract of Pistacia integerrima at doses of 200 mg/kg and 400 mg/kg demonstrated notable tensile strength, with values of 1.92±0.32 kg and 2.15±0.14 kg, respectively. Comparatively, the standard drug Cipladine exhibited a tensile strength of 1.89±0.15 kg.

Wound contraction on the 15th day showed significant improvement for both the standard drug Cipladine (6.3±6.45) and HAPI-treated groups (200mg/kg & 400mg/kg) with wound diameters of 17±3.65 and 4.5±3.47, respectively. Biochemical analysis revealed a substantial increase in hydroxyproline content in the HAPI-treated groups (80.25 & 86.65 µg/gm for 200mg/kg & 400mg/kg, respectively) compared to the disease control and standard drug-treated groups (43.58 & 67.78 µg/gm, respectively). Collagen content was higher in the HAPI-treated groups (601.74 & 635.85 µg/gm for 200mg/kg & 400mg/kg, respectively) compared to the control and standard drug groups (315.47 & 502.32 µg/gm, respectively). Additionally, hexosamine levels in animal tissues were elevated in the HAPI-treated groups (26.65 & 28.74 mg/gm for 200mg/kg & 400mg/kg, respectively) compared to the disease control and standard drug-treated groups (9.65 & 21.45 mg/gm, respectively). In conclusion, the study's results strongly indicate that Pistacia integerrima possesses effective wound healing activity, showcasing its potential as a natural remedy for promoting the recovery process in chronic wounds.