Analysis of Lactic Acid Fermentation by Lactobacillus Bulgaricus NCIM – 2359

Abstract

In this paper the general experimental methods used in the study of   lactic acid fermentation by Lactobacillus bulgaricus NCIM – 2359 has been discussed.  It includes chemical cleaning and steam sterilization of glasswares (fermentor flask, petri-dishes, platinum needle, pipettes and micro-pipettes), preparation and sterilisation of different media such as culture medium, inoculum medium and production medium. Seeding of culture tubes. Inoculation of inoculum medium and production medium. Preparation of buffer solution, incubation of culture tubes, inoculum medium, production medium. Colorimetric determination of lactic acid formed, and molasses (substrate) left unfermented during the course of present investigation.