NMR Analysis of Pharmaceutically Active Secondary Metabolite of Plant Saponin

Abstract

Nuclear Magnetic Resonance (NMR) spectroscopy is (arguably) the most powerful tool available for determining the structure of organic compounds. Saponins are a diverse group of compounds commonly found in legumes, e.g. chick peas, soya beans, lentils, peanuts, lentils, Phaseolus beans and alfalfa sprouts; and in some plants commonly used as flavourings, herbs or spices, e.g. ginseng, fenugreek, sage, quillaja bark, thyme, sarsaparilla and nutmeg. Several biological effects have been ascribed to saponins. Extensive research reported that the membrane-permeabilising, immuno-stimulant, hypocholesterolaemic and anti- carcinogenic properties of saponins and they have also been found to significantly affect growth, feed intake and reproduction in animals. Extraction of saponin from plant calotropis gigeantea were done with the help of methanol as a extracting solvent and analyzed the saponin present in the extract with the help of NMR advance analytical techniques. In which the ¹³C and ¹H analysis done in Cacl3 Solvent and successfully obtained the spectrum data for elucidatation the structure of plant saponin.